PRIMARY ANTIBODY RESPONSE IN ORGAN CULTURES

Primary Antibody Response in Organ Cultures

Specific antibody formation has been elicited in vitro following antigenic stimulation by either sheep (in a total of 472 of 875 cultures) or chick erythrocytes (in 65 of 135 cultures tested). The response was manifested by mouse spleen and lymph node explants whereas thymus cultures were inactive. The reaction has been characterized as a primary immune response in view of its kinetics as compa...

Antibody production in organ cultures of lymph-node fragments following in vitro secondary antigenic stimulation.

Primary Antibody Response I N Peritoneal Cells

Since the early days of immunology many workers have tried to initiate and maintain in vitro an immunological response leading to the formation of antibody by cells from nonimmune animals (1, 2). This quest has been revived in the last decade, since immunologists felt the need of such an approach at a cellular level. Various laboratories have succeeded ir maintaining tissues and cells actively ...

Using elicitors for enhanced production of secondary metabolites in plant cell and organ suspension cultures

In biotechnology has been concentrated to review alternative routes for the production of natural compounds. Plant cell culture systems are viable alternatives for the production of secondary metabolites that are of commercial importance in food and pharmaceutical industries. However, relatively very few cultures synthesize these compounds over extended periods in amounts comparable to those fo...

Development of Fetal Thymocytes in Organ Cultures

When mature thymus-derived (T) lymphocytes from peripheral lymphoid organs are activated by antigen or mitogen, an increase in the expression of the surface receptor for interleukin 2 (IL-2-R) is observed that precedes the development of effector function (1-3). While IL-2 is known to be a specific growth factor for such mature T cells (4, 5), it is not known whether IL-2 is also involved as a ...